Factors Affecting the Sensitivity of Limulus Lysate
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چکیده
منابع مشابه
Biomedical Applications of Limulus Amebocyte Lysate
This year celebrates the 30th anniversary of the licensing of Limulus amebocyte lysate (LAL) by the US Food and Drug Administration (FDA) as a test for the presence of endotoxin in biologicals, pharmaceutical drugs, and medical devices. LAL is currently recognized by several major pharmacopoeias and is used worldwide. That a suitable alternative for the detection of endotoxin has not supplanted...
متن کاملModified micromethod of the Limulus amoebocyte lysate assay for endotoxin.
A Limulus amoebocyte lysate microtechnique performed in petrolatum wells on a microscope slide is described. Injection of a dye solution in ethanol directly into the wells leads to an unambiguous interpretation of the results. Twelve samples can be tested on a single slide, and compact storing of the samples is possible.
متن کاملEffect of semisynthetic penicillins on the Limulus lysate test.
Semisynthetic penicillins in concentrations greater than 1.0% (wt/vol) were found to inhibit the limulus lysate assay for endotoxin.
متن کاملEndotoxins and Their Detection with the Limulus Amebocyte Lysate Test
In contrast to the detailed descriptions of techniques provided by Larsson, a chapter on the measurement and analysis of acetylcholine written by Israel Hanin takes the form of a brief but comprehensive survey of all of the methods for the quantitation of acetylcholine introduced between 1933 and 1981. Each method presented is referenced and briefly critiqued. This is followed by an article by ...
متن کاملRapid determination of bacteriological water quality by using limulus lysate.
The Limulus lysate assay was used to measure the endotoxin content in stream water and was found to reflect the degree of bacterial contamination as measured by coliform, enteric, gram-negative, and heterotrophic bacteria. The firm-clot method was found to be a less sensitive and reproducible technique for the detection of endotoxin than was the spectrophotometric modification of the Limulus ly...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1974
ISSN: 0003-6919
DOI: 10.1128/am.28.6.1023-1026.1974